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Urine metabonomic study on long-term use of total ginsenosides in rats
XIE Xie1， CHEN Shao-qiu2， LV Ying-fang1， WANG Xiao-yan1*， JIA Wei1
（1.Ministry of Education Key Laboratory of Systems Biomedicine， Shanghai Center for Systems Biomedicine，
Shanghai Jiao Tong University， Shanghai 200240， China；
2.Anhui Agriculture University， Hefei 230036， China）
[Abstract] Due to its effect of systems regulation and promotion on body， Ginseng is always referred to be long-term used as a dietary supplement. But it was still unclear about its target of the tonic effects and also the side-effects long-term use may bring. Urine metabolomic method is suitable for long-term studies of pharmaco-dynamics， pharmacology and toxicology of traditional Chinese medicine because of its characteristics of non-invasive and monitoring the whole-body metabolism. This study was designed to detect the dynamic variation of rat urine metabolome along with a long-term administration of total ginsenosides using GC-TOF based metabolomic technology. Our result showed that either short-term or chronic administration of ginsenosides did not impact the rat urine metabolome significantly （as the PCA subgroup was not successful）. By comparison， the short-term（1-3 w） dose of ginsenosides had the biggest metabolic influence including TCA cycle， catecholamines and neurotransmitter amino acids. Medium-term （6-10 w） dose had a gradually lower effect and long-term （27 w） dose almost had no effect. Our study indicates that both short and long term administration of ginsenosides showed almost no obvious side-effect on the experimental animals.
[Key words] ginsenoside； urine； metabolomics
doi：10.4268/cjcmm20142337
[責任編輯 張燕]