青壯年猝死綜合征腦幹中縫背核5-羥色胺及5-羥色胺轉運體免疫組化研究
病理與臨床
作者：吳華 唐群 瞿誌雄 王健 王興華
[摘要] 目的 探討5-羥色胺和5-羥色胺轉運體在青壯年猝死綜合征腦組織的表達及其意義。 方法 運用免疫組織化學S-P法檢測10例正常腦組織和8例青壯年猝死綜合征腦組織中5-羥色胺和5-羥色胺轉運體的表達水平，利用圖像分析技術測量陽性細胞的平均光密度值。 結果 青壯年猝死征死者腦幹中縫背核5-羥色胺的平均光密度值低於正常腦組織，而5-羥色胺轉運體的平均光密度值高於正常腦組織，差異均有統計學意義（P＜0.05）。結論 5-羥色胺及5-羥色胺轉運體的變化參與青壯年猝死綜合征的發病過程，可作為診斷青壯年猝死綜合征的參考指標。
[關鍵詞] 法醫病理學；青壯年猝死綜合征；5-羥色胺；5-羥色胺轉運體；免疫組化
[中圖分類號] RF795.1[文獻標識碼] B[文章編號] 1673-9701（2014）15-0061-02
Immunohistochemical study on SMDS dorsal nucleus raphe 5-serotonin and 5-serotonin transporter
WU Hua1 TANG Qun2 QU Zhixiong1WANG Jian1 WANG Xinghua1
1.Furong Judicial Identification Center of the Second People's Hospital of Hunan Province, Changsha 410007,China；2. Department of Pathology of Hunan University of Chinese Medicine, Changsha 410208, China
[Abstract] Objective To investigate the 5- serotonin and 5-serotonin transporter expression and significance in brain tissue of sudden manhood death syndrome. Methods Used immunohistochemical S-P staining method in 10 cases of normal brain tissues and 8 cases of sudden manhood death syndrome in expression of 5-HT and 5-serotonin transporter in the brain tissue, using image analysis technique to measure the mean optical density of positive cells of value. Results The average optical density of the 5- serotonin in the dorsal nucleus raphe of sudden manhood death syndrome was lower than that in normal brain tissue, and the average optical density of 5-serotonin transporter was higher than that in normal brain tissue, the differences were statistically significant(P
[Key words] Forensic pathology; Sudden manhood death syndrome; 5-serotonin; 5-serotonin transporter; Immunohistochemistry本實驗通過觀察青壯年猝死征（sudden manhood death syndrome ，SMDS）死者腦幹中縫背核中5-羥色胺（5-ydroxytryptamine，5-HT）及5-羥色胺轉運體（5-HT transporter，5-HTT/ serotonin transporter，SERT）的表達變化，探討5-HT、SERT與SMDS之間的關係，以期為SMDS的死因分析研究提供實驗依據。